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tpc-1 bcpap human papillary thyroid cancer cell lines  (Procell Inc)

 
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    Procell Inc tpc-1 bcpap human papillary thyroid cancer cell lines
    Tpc 1 Bcpap Human Papillary Thyroid Cancer Cell Lines, supplied by Procell Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tpc-1+bcpap+human+papillary+thyroid+cancer+cell+lines/tpc+1+cells/pm39450935-15-62-71
    Average 90 stars, based on 1 article reviews
    tpc-1 bcpap human papillary thyroid cancer cell lines - by Bioz Stars, 2026-10
    90/100 stars

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    Article Title: Human adipose-derived stem cells promote migration of papillary thyroid cancer cell via leptin pathway.
    Article Snippet: Cell lines, flow cytometry, and cell culture aDscs (catalog# hUXMD-01001) used in this study were obtained from cyagen Biosciences, inc. expression of surface markers was analyzed using fluorescence-labelled antibodies, followed by analysis on a Facscanto ii flow cytometer (BD Biosciences, Usa). antibodies against cD11b, cD29, cD34, cD44, cD45, cD73, and cD105 (catalog numbers 347557, 556049, 555822, 566803, 555489, 550257, 560839, BD, Usa, respectively) were used for staining. .. Fcs express 7 software (De Novo software, canada) was used for statistical analysis and visualization. cells were verified to be positive for cD105, cD29, cD73 and cD44, and negative for cD34, cD45, and cD11b (Figure s1). cells were cultured and passaged in adipose-derived mesenchymal stem cell complete medium (cyagen Biosciences, inc.), and 4-8 passages of cells were used in the experiments. the tPc-1 and BcPaP human papillary thyroid cancer cell lines (Procell life science & technology, co., ltd.) were cultured in RPMi-1640 medium (thermo Fisher scientific, inc.) containing 10% fetal bovine serum [15,16]. .. the aDscs were grown to 70 to 90% confluence in adipose-derived mesenchymal stem cell complete medium. the medium was then replaced with RPMi-1640 medium containing 1% fetal bovine serum (Gibco). the conditioned medium of aDscs (aDsc-cM) was collected after culturing for 1 week. the conditioned medium was filtered through a 0.22-μm syringe filter (Milliporesigma) to remove cellular debris and used to culture the tPc-1/BcPaP cells after the addition of 5% fetal bovine serum. the complete culture medium of adipose mesenchymal stem cells cultured with tPc-1/BcPaP cells was used as a negative control.

    Cell Culture:

    Article Title: Human adipose-derived stem cells promote migration of papillary thyroid cancer cell via leptin pathway.
    Article Snippet: Cell lines, flow cytometry, and cell culture aDscs (catalog# hUXMD-01001) used in this study were obtained from cyagen Biosciences, inc. expression of surface markers was analyzed using fluorescence-labelled antibodies, followed by analysis on a Facscanto ii flow cytometer (BD Biosciences, Usa). antibodies against cD11b, cD29, cD34, cD44, cD45, cD73, and cD105 (catalog numbers 347557, 556049, 555822, 566803, 555489, 550257, 560839, BD, Usa, respectively) were used for staining. .. Fcs express 7 software (De Novo software, canada) was used for statistical analysis and visualization. cells were verified to be positive for cD105, cD29, cD73 and cD44, and negative for cD34, cD45, and cD11b (Figure s1). cells were cultured and passaged in adipose-derived mesenchymal stem cell complete medium (cyagen Biosciences, inc.), and 4-8 passages of cells were used in the experiments. the tPc-1 and BcPaP human papillary thyroid cancer cell lines (Procell life science & technology, co., ltd.) were cultured in RPMi-1640 medium (thermo Fisher scientific, inc.) containing 10% fetal bovine serum [15,16]. .. the aDscs were grown to 70 to 90% confluence in adipose-derived mesenchymal stem cell complete medium. the medium was then replaced with RPMi-1640 medium containing 1% fetal bovine serum (Gibco). the conditioned medium of aDscs (aDsc-cM) was collected after culturing for 1 week. the conditioned medium was filtered through a 0.22-μm syringe filter (Milliporesigma) to remove cellular debris and used to culture the tPc-1/BcPaP cells after the addition of 5% fetal bovine serum. the complete culture medium of adipose mesenchymal stem cells cultured with tPc-1/BcPaP cells was used as a negative control.



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